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ref: -2021 tags: synaptic imaging weights 2p oregon markov date: 12-29-2021 23:30 gmt revision:2 [1] [0] [head]

Distinct in vivo dynamics of excitatory synapses onto cortical pyramidal neurons and parvalbumin-positive interneurons

  • Joshua B.Melander, Aran Nayebi, Bart C.Jongbloets, Dale A.Fortin, Maozhen Qin, Surya Ganguli, Tianyi Mao, Haining Zhong
  • Cre-dependent mVenus labeled PSD-95, in both excitatory pyramidadl neurons & inhibitory PV interneurons.
  • morphology labeled with tdTomato
  • Longitudinal imaging of individual exictatoy post-synaptic densityies; estimated weight from fluorescence; examined spine appearance and disappearance
  • PV synapses were more stable over the 24-day period than synapses on pyramidal neurons.
  • Likewise, large synapses were more likely to remain over the imaging period.
  • Both followed log-normal distributions in 'strengths'
  • Changes were well modeled by a Markov process, which puts high probability on small changes.
  • But these changes are multiplicative (+ additive component in PV cells)