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ref: Biran-2005.09 tags: Tresco histology chronic implantation astrocytes microglia date: 01-04-2013 02:28 gmt revision:3 [2] [1] [0] [head]

PMID-16045910[0] Neuronal cell loss accompanies the brain tissue response to chronically implanted silicon microelectrode arrays.

  • We observed persistent ED1 immunoreactivity around implanted silicon microelectrode arrays implanted in adult rat cortex that was accompanied by a significant reduction in nerve fiber density and nerve cell bodies in the tissue immediately surrounding the implanted silicon microelectrode arrays.
  • We found that explanted electrodes were covered with ED1/MAC-1 immunoreactive cells and that the cells released MCP-1 and TNF-a under serum-free conditions in vitro.
  • See also [1] and [2]
  • Electrodes: Michigan type, 5mm long, 200um wide tapering to 30um, 15um thick at the shank tapering to 2um.
    • Show that the chronic response is markedly different than acute stab wounds.
    • "Stab wounds resulted in comparatively minimal neurofilament loss at 2 weeks (A) and no apparent loss by 4 weeks".
    • "The number of neuronal bodies is reduced in the area adjacent to microelectrodes (B, D) but appears unaltered surrounding stab wound lesions (A, C; lesion site in center of each image)."
  • Includes details of immunostaining, which could be useful.

____References____

[0] Biran R, Martin DC, Tresco PA, Neuronal cell loss accompanies the brain tissue response to chronically implanted silicon microelectrode arrays.Exp Neurol 195:1, 115-26 (2005 Sep)
[1] Szarowski DH, Andersen MD, Retterer S, Spence AJ, Isaacson M, Craighead HG, Turner JN, Shain W, Brain responses to micro-machined silicon devices.Brain Res 983:1-2, 23-35 (2003 Sep 5)
[2] Gilletti A, Muthuswamy J, Brain micromotion around implants in the rodent somatosensory cortex.J Neural Eng 3:3, 189-95 (2006 Sep)