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ref: -2013 tags: 2p two photon STED super resolution microscope synapse synaptic plasticity date: 08-14-2020 01:34 gmt revision:3 [2] [1] [0] [head]

PMID-23442956 Two-Photon Excitation STED Microscopy in Two Colors in Acute Brain Slices

  • Plenty of details on how they set up the microscope.
  • Mice: Thy1-eYFP (some excitatory cells in the hippocampus and cortex) and CX3CR1-eGFP (GFP in microglia). Crossbred the two strains for two-color imaging.
  • Animals were 21-40 days old at slicing.

PMID-29932052 Chronic 2P-STED imaging reveals high turnover of spines in the hippocampus in vivo

  • As above, Thy1-GFP / Thy1-YFP labeling; hence this was a structural study (for which the high resolution of STED was necessary).
  • Might just as well gone with synaptic labels, e.g. tdTomato-Synapsin.