PMID-22542188 Clustered dynamics of inhibitory synapses and dendritic spines in the adult neocortex.
- Cre-recombinase-dependent labeling of postsynapitc scaffolding via Gephryn-Teal fluorophore fusion.
- Also added Cre-eYFP to label the neurons
- Electroporated in utero e16 mice.
- Low concentration of Cre, high concentrations of Gephryn-Teal and Cre-eYFP constructs to attain sparse labeling.
- Located the same dendrite imaged in-vivo in fixed tissue - !! - using serial-section electron microscopy.
- 2230 dendritic spines and 1211 inhibitory synapses from 83 dendritic segments in 14 cells of 6 animals.
- Some spines had inhibitory synapses on them -- 0.7 / 10um, vs 4.4 / 10um dendrite for excitatory spines. ~ 1.7 inhibitory
- Suggest that the data support the idea that inhibitory inputs maybe gating excitation.
- Furthermore, co-inervated spines are stable, both during mormal experience and during monocular deprivation.
- Monocular deprivation induces a pronounced loss of inhibitory synapses in binocular cortex.
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