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ref: -0 tags: two photon scanning microscope mirror relay date: 01-31-2020 02:46 gmt revision:1 [0] [head]

PMID-24877017 Optimal lens design and use in laser-scanning microscopy

  • Detail careful design of a scanning two-photon microscope, with custom scan lens, tube lens, and standard 25x objective.
  • Near diffraction limited performance for both the scan and tube lenses across a broad excitation range -- 690 to 1400nm.
  • Interestingly, use a parabolic mirror relay to conjugate the two galvos to each other; seems like a good idea, why has this not been done elsewhere?

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ref: -0 tags: superresolution imaging scanning lens nanoscale date: 02-04-2019 20:34 gmt revision:1 [0] [head]

PMID-27934860 Scanning superlens microscopy for non-invasive large field-of-view visible light nanoscale imaging

  • Recently, the diffraction barrier has been surpassed by simply introducing dielectrics with a micro-scale spherical configuration when using conventional optical microscopes by transforming evanescent waves into propagating waves. 18,19,20,21,22,23,24,25,26,27,28,29,30
  • The resolution of this superlens-based microscopy has been decreased to ∼50 nm (ref. 26) from an initial resolution of ∼200 nm (ref. 21).
  • This method can be further enhanced to ∼25 nm when coupled with a scanning laser confocal microscope 31.
  • It has achieved fast development in biological applications, as the sub-diffraction-limited resolution of high-index liquid-immersed microspheres has now been demonstrated23,32, enabling its application in the aqueous environment required to maintain biological activity.
  • Microlens is a 57 um diameter BaTiO3 microsphere, resolution of lambda / 6.3 under partial and inclined illumination
  • Microshpere is in contact with the surface during imaging, by gluing it to the cantilever tip of an AFM.
  • Get an image with the microsphere-lens, which improves imaging performance by ~ 200x. (with a loss in quality, naturally).