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ref: -0 tags: DNA paint FRET tag superresolution imaging oligos date: 02-20-2020 16:28 gmt revision:1 [0] [head]

Accelerated FRET-PAINT Microscopy

  • Well isn't that smart -- they use a FRET donor, which is free to associate and dissociate form a host DNA strand, and a more-permanently attached DNA acceptor, which blinks due to FRET, for superresolution imaging.
  • As FRET acceptors aren't subject to bleaching (or, perhaps, much less subject to bleaching), this eliminates that problem...
  • However, the light levels used ~1kW / cm^2, does damage the short DNA oligos, which interferes with reversible association.
  • Interestingly, CF488 donor showed very little photobleaching; DNA damage was instead the limiting problem.
    • Are dyes that bleach more slowly better at exporting their singlet oxygen (?) or aberrant excited states (?) to neighboring molecules?

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ref: -0 tags: adhesion polymer metal FTIR epoxy eponol paint date: 05-01-2015 19:20 gmt revision:0 [head]

Degradation of polymer/substrate interfaces – an attenuated total reflection Fourier transform infrared spectroscopy approach

  • Suggests why eponol is used as an additive to paint.
  • In this thesis, attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy has been used to detect changes at the interfaces between poly (vinyl butyral-co-vinyl alcohol-co-vinyl acetate) (PVB) and ZnSe upon exposure to ozone, humidity and UV-B light.
  • Also, the response of PVB-aluminum interfaces to liquid water has been studied and compared with the same for eponol (epoxy resin, diglycidyl ether of bisphenol A)-aluminum interfaces.
  • In the presence of ozone, humidity and UV-B radiation, an increase in carbonyl group intensity was observed at the PVB-ZnSe interface indicating structural degradation of the polymer near the interface. However, such changes were not observed when PVB coated ZnSe samples were exposed to moisture and UV-B light in the absence of ozone showing that ozone is responsible for the observed structural deterioration. Liquid water uptake kinetics for the degraded PVB monitored using ATR-FTIR indicated a degradation of the physical structural organization of the polymer film.
  • Exposure of PVB coated aluminum thin film to de-ionized water showed water incorporation at the interface. There were evidences for polymer swelling, delamination and corrosion of the aluminum film under the polymer layer.
    • On the contrary, delamination/swelling of the polymer was not observed at the eponol-aluminum interface, although water was still found to be incorporated at the interface. Al-O species were also observed to form beneath the polymer layer.
    • A decrease of the C-H intensities was detected at the PVB-aluminum interface during the water uptake of the polymer, whereas an increase of the C-H intensities was observed for the eponol polymer under these conditions.
    • This is assigned to rearrangement of the macromolecular polymer chains upon interaction with water.

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ref: bookmark-0 tags: goodbrush paintings favorite date: 11-24-2008 03:03 gmt revision:4 [3] [2] [1] [0] [head]

hopefully these links don't move..

I like these 'paints', too. Did you show them to me a long time ago? I remember someone showing them to me in the past and I am wondering if you were the one. -- Ana

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ref: notes-0 tags: software debian xpaint maxima math mathematica date: 07-02-2008 14:37 gmt revision:3 [2] [1] [0] [head]

oldies but goodies:

  • Maxima a computer algebra system, almost like a free version of Mathematica!
    • be sure to install maxima-emacs to get LaTeX prettyprinting.
  • [xpaint] Has a cool spring-mass-friction system where the length of the spring (the distance between cursor and paint brush) controls the width of the paint brush. see below!

Both are in Debian of course :)