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{1498}
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ref: -0 tags: lavis jf dyes fluorine zwitterion lactone date: 01-22-2020 20:06 gmt revision:0 [head]

Optimization and functionalization of red-shifted rhodamine dyes

  • Zwitterion form is fluorescent and colored; lactone form is not and colorless.
  • Lactone form is lipophyllic; some mix seems more bioavailable and also results in fluorogenic dyes.
  • Good many experiments with either putting fluorine on the azetidines or on the benzyl ring.
  • Fluorine on the azetidine pushes the K ZLK_{Z-L} toward lactone form; fluorine on the benzyl ring pushes it toward the zwitterion.
  • Si-rhodamine and P-rhodamine adopt the lactone form, and adding appropriate fluorines can make them fluorescent again. Which makes for good red-shifted dyes, ala JF669
  • N-CH3 can be substituted in the oxygen position too, resulting in blue-shifted dye which is a good stand-in for EGFP.

{1349}
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ref: -0 tags: bone regrowth hyperelastic 3d print implant hydroxyapatite polycaptolactone date: 09-30-2016 18:27 gmt revision:0 [head]

Hyperelastic “bone”: A highly versatile, growth factor–free, osteoregenerative, scalable, and surgically friendly biomaterial

  • (From the abstract): hyperelastic “bone” is composed of 90 weight % (wt %) hydroxyapatite and 10 wt % polycaprolactone or poly(lactic-co-glycolic acid),
  • Can be rapidly three-dimensionally (3D) printed (up to 275 cm3/hour) from room temperature extruded liquid inks.
  • Mechanical properties: ~32 to 67% strain to failure, ~4 to 11 MPa elastic modulus & was highly absorbent (50% material porosity)
  • Supported cell viability and proliferation, and induced osteogenic differentiation of bone marrow–derived human mesenchymal stem cells cultured in vitro over 4 weeks without any osteo-inducing factors in the medium.
  • HB did not elicit a negative immune response, became vascularized, quickly integrated with surrounding tissues, and rapidly ossified and supported new bone growth without the need for added biological factors.

{746}
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ref: Sanders-2000.1 tags: polymer fiber immune reaction biocompatibility rats polycaprolactone recording electrodes histology MEA date: 01-28-2013 00:01 gmt revision:11 [10] [9] [8] [7] [6] [5] [head]

PMID-10906696[0] Tissue response to single-polymer fibers of varying diameters: evaluation of fibrous encapsulation and macrophage density.

  • Fibers smaller than 6μm6 \mu m show reduced immune response.
    • Fibers implanted in the subcutaneous dorsum (below the skin in the back of rats).
    • Polypropylene. (like rope).
    • Wish the result extended to small beads & small electrodes. 7μm7 \mu m is tiny, but possible with insulated Au wires.
      • Beads: try PMID-1913150 -- shows that the 600um - 50um beads ('microspheres') are well tolerated.
      • Also {750}.
  • Macrophage density in tissue with fiber diameters 2.1-5.9um comparable to that of unoperated contralateral control.

"

fiber diametercapsule thickness
2.1-5.90.6
6.5-10.611.7
11.1-15.820.3
16.7-26.725.5

____References____

[0] Sanders JE, Stiles CE, Hayes CL, Tissue response to single-polymer fibers of varying diameters: evaluation of fibrous encapsulation and macrophage density.J Biomed Mater Res 52:1, 231-7 (2000 Oct)

{750}
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ref: Menei-1994.09 tags: microspheres beads polycaprolactone biocompatible drug delivery histology date: 01-27-2013 20:54 gmt revision:3 [2] [1] [0] [head]

PMID-7814435 Fate and biocompatibility of three types of microspheres implanted into the brain.

  • microspheres ( 24μm 24 \mu m ) appear to be engulfed or surrounded by histocytic cells.
  • poly(e-caprolactone), which is supposed to be biodegradable, did not dissolve in the brain. The polymer is hydrophobic.
  • 20um spheres could be engulfed by macrophages; their microspheres were too large, and were encapsulated in a thin coallagen layer and astrocytic process.
  • no scale bars - annoying - but we can estimate the size of the coating to be about the same size as the beads themselves.