PMID-23900251 Parallel super-resolution imaging
- Christopher J Rowlands, Elijah Y S Yew, and Peter T C So
- Though this is a brief Nature intro article, I found it to be more usefully clear than the wikipedia articles on super-resolution techniques.
- STORM and PALM seek to stochastically switch fluorophores between emission and dark states, and are parallel but stochastic; STED and RESOLFT use high-intensity donut beams to stimulate emission (STED) or photobleach (RESOLFT) fluorophores outside of an arbitrarily-small location.
- All need gaussian-fitting to estimate emitter location from the point-spread function.
- This article comments on a clever way of making 1e5 donuts for parallel (as opposed to rastered) STED / RESOLFT.
- I doubt stetting up a STED microscope is at all easy; to get these resolutions, everything must be still to a few nm!
|