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ref: -2011 tags: two photon cross section fluorescent protein photobleaching Drobizhev date: 12-05-2019 17:04 gmt revision:1 [0] [head]

PMID-21527931 Two-photon absorption properties of fluorescent proteins

  • Significant 2-photon cross section of red fluorescent proteins (same chromophore as DsRed) in the 700 - 770nm range, accessible to Ti:sapphire lasers ...
    • This corresponds to a S 0S nS_0 \rightarrow S_n transition
    • But but, photobleaching is an order of magnitude slower when excited by the direct S 0S 1S_0 \rightarrow S_1 transition (but the fluorophores are significantly less bright in this regime).
    • See also PMID-18027924
  • 2P cross-section in the 1000-1100 nm range corresponds to the chromophore polarizability, and is not related to 1p cross section.